Extraction and characterization of native canine bone morphogenetic protein (cBMP) qualified with osteoinductivity.

Bone morphogenetic protein (BMP) was extracted from canine bone matrix, partially purified and tested for osteoinductivity. A radiographically and histologically detectable ectopic bone formation was induced by 6.0 mg canine (cBMP) in muscle pouch of BALB mouse at 21 days post implantation. Characterization of the cBMP preparation by a gel filtration chromatography defined that the material consisted of proteins or protein complexes with molecular weights from 4 to 120 kD. Isoelectric focusing showed that the molecules were acidic with isoelectric points of 4.6-5.6.

[The use of bone morphogenetic proteins in delayed fracture healing, pseudoarthrosis and in ulna osteotomy carried out because of elbow joint diseases]. / Die Anwendung knochenmorphogenetischen Proteins bei verzögerter Frakturheilung, Pseudarthrose und bei wegen Ellbogengelenkerkrankungen durchgeführter Ulnaosteotomie.

This study deals with the application of bone morphogenetic protein (BMP) as an osteoinductive factor in the treatment of fractures and elbow disease in the dog. Partially purified canine bone morphogenetic protein (cBMP) was used in the repair of a delayed union fracture and a pseudoarthrosis. The cBMP was applied in Biocoral and Tricalciumphosphate carriers using a subcortical grafting method. Two dogs suffering from incongruence and subluxation of the elbow joint were treated with a partially purified bone morphogenetic protein of moose bone origin placed in the gap produced by ulnar osteotomy.

Structural variation in the nasal bone region of European moose (Alces alces L.).

The ontogeny of typical (normal) nasal bone region of the European moose (Alces alces L.) and the 3 variants of the pattern, was studied. The variants, named as "extra bone type", "punctured type" and "open type" referring the morphology of the internasal suture, were originally observed in Finnish male and female moose skulls in 1971. All of the variants were later found in hunting trophy exhibitions presenting male moose trophies from Sweden, Norway, Baltic Republics of USSR and Poland. The frequencies of the variants showed regional differences. By using histological, radiological and OTC bone labelling methods, all of the nasal bone types were observed in this study in embryonal (N = 36), newborn (N = 21), juvenile (N = 38) and adult (N = 12) moose. Two twin embryos showed different nasal bone structure. The variation is considered to be of congenital origin.

[Dysostoses and postural limb abnormalities in rapidly growing dogs]. / Dysostosen und Stellungsfehler der Gliedmassen bei Hunden mit hoher Wachstumsintensität.

Congenital and acquired dysostosis was studied on two litters of Great Danes. One litter of puppies were descendants of dogs with normal hips, the other of dysplastic animals. Both litters were fed according to NCR standards, the second however ad libitum. One puppy of parents with normal hips demonstrated already with 6 weeks epi- and metaphyseal dysostosis and severe angular deformities of the thoracic and pelvic limbs. Hip dysplasia was diagnosed in three of five puppies from dysplastic dogs. In addition, one puppy showed Wobbler symptoms. The extent and frequency of the alterations were, in comparison to earlier investigations, unexpectedly high. It can be assumed that the examined dogs were genetically predisposed for dysostosis. Our therapeutic procedures are presented.

Etiological factors in osteochondritis dissecans. An experimental study into the etiological factors in osteochondritis dissecans in the canine humeral head using overloading with and without somatotropin and thyrotropin hormone treatment and mechanical trauma.

The overloaded humeral heads of the six growing Labrador retrievers treated with somatotropin and thyrotropin hormone showed the most pronounced changes of the articular cartilage and subchondral home, resembling changes in the early stage of spontaneous osteochondritis dissecans. The overloading alone used in 16 dogs without hormone treatment produced no changes, except that two Labrador retrievers showed increased radiodensity subchondrally during the first 4-6 weeks of the observation period. The mechanical traumas of the articular cartilage and the epiphyseal bone regenerated and did not result in changes resembling those of spontaneous osteochondritis dissecans.